Composite

Part:BBa_K1651000:Design

Designed by: YANG YUANZHAN   Group: iGEM15_BIT   (2015-09-13)

PAI1 detector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 987
    Illegal NheI site found at 2604
    Illegal NheI site found at 2627
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1773
    Illegal BamHI site found at 2901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3376
    Illegal BsaI.rc site found at 2513


Design Notes

There are two systems including LasIR and RhlIR existing in the bacterium. So what is considered about is to make a streamline design of the two-stage amplification element. Meanwhile, the core regulatory element is also restained.


Source

Series group effect element P.Aeruginosa.

References